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Eliminating caspase-7 and cathepsin B cross-reactivity on fluorogenic caspase-3 substrates
11 FRET-based fluorogenic substrates were constructed using the pentapeptide template Asp-Glu-X(2)-Asp-X(1)′, and evaluated with caspase-3, caspase-7 and cathepsin B. The sequence Asp-Glu-Pro-Asp-Ser was able to selectively quantify caspase-3 activity in vitro without notable caspase-7 and cathepsin...
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| Publicado no: | Mol Biosyst |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Royal Society of Chemistry
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4763880/ https://ncbi.nlm.nih.gov/pubmed/26726961 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5mb00730e |
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