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CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei
The limited repertoire of drug-resistance markers imposes a serious obstacle to genetic manipulation of Trypanosoma brucei. Here we describe experiments with a fusion protein that allows positive selection for genome integration followed by CRE recombinase-mediated excision of the marker cassette th...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2007
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2211722/ https://ncbi.nlm.nih.gov/pubmed/18006158 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2007.10.003 |
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