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Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein
We report the design and engineering of a robust, reagentless fluorescent glucose biosensor based on the periplasmic glucose-binding protein obtained from Thermotoga maritima (tmGBP). The gene for this protein was cloned from genomic DNA and overexpressed in Escherichia coli, the identity of its cog...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2204141/ https://ncbi.nlm.nih.gov/pubmed/17766373 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.072969407 |
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