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Redesigning protein pK(a) values

The ability to re-engineer enzymatic pH-activity profiles is of importance for industrial applications of enzymes. We theoretically explore the feasibility of re-engineering enzymatic pH-activity profiles by changing active site pK(a) values using point mutations. We calculate the maximum achievable...

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Detalhes bibliográficos
Main Authors: Tynan-Connolly, Barbara Mary, Nielsen, Jens Erik
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2203286/
https://ncbi.nlm.nih.gov/pubmed/17189477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062538707
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