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Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis

Raucaffricine glucosidase (RG) is an enzyme that is specifically involved in the biosynthesis of indole alkaloids from the plant Rauvolfia serpentina. After heterologous expression in Escherichia coli cells, crystals of RG were obtained by the hanging-drop vapour-diffusion technique at 293 K with 0....

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Ruppert, Martin, Panjikar, Santosh, Barleben, Leif, Stöckigt, Joachim
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: International Union of Crystallography 2006
Θέματα:
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2197181/
https://ncbi.nlm.nih.gov/pubmed/16511316
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910600457X
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