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Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis

Raucaffricine glucosidase (RG) is an enzyme that is specifically involved in the biosynthesis of indole alkaloids from the plant Rauvolfia serpentina. After heterologous expression in Escherichia coli cells, crystals of RG were obtained by the hanging-drop vapour-diffusion technique at 293 K with 0....

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Bibliografiske detaljer
Main Authors: Ruppert, Martin, Panjikar, Santosh, Barleben, Leif, Stöckigt, Joachim
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2197181/
https://ncbi.nlm.nih.gov/pubmed/16511316
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910600457X
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