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Colorimetric Determination of Pure Mg(2+)-dependent Phosphatidate Phosphatase Activity
The malachite green-molybdate reagent was used for a colorimetric assay of pure Mg(2+)-dependent phosphatidate phosphatase activity. This enzyme plays a major role in fat metabolism. Enzyme activity was linear with time and protein concentration, and with the concentration of water-soluble dioctanoy...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2194655/ https://ncbi.nlm.nih.gov/pubmed/17910939 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.08.037 |
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