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A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in Unperturbed Dividing Yeast Cells

BACKGROUND: Imaging single cells with fluorescent markers over multiple cell cycles is a powerful tool for unraveling the mechanism and dynamics of the cell cycle. Over the past ten years, microfluidic techniques in cell biology have emerged that allow for good control of growth environment. Yet the...

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Detalles Bibliográficos
Main Authors: Charvin, Gilles, Cross, Frederick R., Siggia, Eric D.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2008
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2194624/
https://ncbi.nlm.nih.gov/pubmed/18213377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0001468
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