F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.

We describe the construction and properties of an F' factor which carries the temperature-sensitive cI857 allele of the repressor gene of coliphage lambda and which lacks the lambda cro function. This episome can easily be transferred to any F- and F' Escherichia coli strain, thus facilita...

Full description

Saved in:
Bibliographic Details
Main Authors: Mieschendahl, M, Müller-Hill, B
Format: Artigo
Language:Inglês
Published: 1985
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC219341/
https://ncbi.nlm.nih.gov/pubmed/2999084
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items