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Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts.

A procedure for efficient transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts with plasmid DNA was developed. Transformation frequencies with S. fradiae protoplasts were strongly influenced by the temperatures for cell growth, protoplast formation, and protoplast regenera...

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Bibliographic Details
Main Authors: Matsushima, P, Baltz, R H
Format: Artigo
Language:Inglês
Published: 1985
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC219096/
https://ncbi.nlm.nih.gov/pubmed/4008440
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