Transformation system for Amycolatopsis (Nocardia) mediterranei: direct transformation of mycelium with plasmid DNA.
A new procedure for transformation of Amycolatopsis (Nocardia) mediterranei LBG A3136 was developed. The method makes use of polyethylene glycol and alkaline cations and enables direct transformation of the A. mediterranei mycelium with high efficiency: more than 10(6) transformants per microgram of...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208963/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1655706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.20.6325-6331.1991 |
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