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Transformation system for Amycolatopsis (Nocardia) mediterranei: direct transformation of mycelium with plasmid DNA.

A new procedure for transformation of Amycolatopsis (Nocardia) mediterranei LBG A3136 was developed. The method makes use of polyethylene glycol and alkaline cations and enables direct transformation of the A. mediterranei mycelium with high efficiency: more than 10(6) transformants per microgram of...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Principais autores: Madoń, J, Hütter, R
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1991
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208963/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1655706/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.20.6325-6331.1991
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