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Separation of antigen-specific lymphocytes. I. Enrichment of antigen- binding cells
Normal mouse spleen cells were fractionated in dishes coated with thin layers of DNP-gelatin or NIP-gelatin, which were insoluble at 4 degrees C. Highly viable cells were recovered from the dishes by melting the gel at 37 degrees C. NIP3- gelatin layers bound approximately 0.1% and DNP4-gelatin laye...
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| Format: | Artigo |
|---|---|
| Sprog: | Inglês |
| Udgivet: |
The Rockefeller University Press
1975
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2189776/ https://ncbi.nlm.nih.gov/pubmed/47891 |
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