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Enrichment of antigen-specific B lymphocytes by the direct removal of B cells not bearing specificity for the antigen
Antigen-specific B cells (ASC) were purified from other B cells by prior incubation with specific antigen followed by rosetting with erythrocytes conjugated with anti-mouse Ig and sedimenting on Ficoll- Isopaque. This procedure allowed the removal of most of the B cells, while those speicifc for the...
Gardado en:
| Formato: | Artigo |
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| Idioma: | Inglês |
| Publicado: |
The Rockefeller University Press
1977
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2180762/ https://ncbi.nlm.nih.gov/pubmed/69002 |
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