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Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures
Green fluorescent protein has revolutionized cell labeling and molecular tagging, yet the driving force and mechanism for its spontaneous fluorophore synthesis are not established. Here we discover mutations that substantially slow the rate but not the yield of this posttranslational modification, d...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC218721/ https://ncbi.nlm.nih.gov/pubmed/14523232 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2133463100 |
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