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Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures

Green fluorescent protein has revolutionized cell labeling and molecular tagging, yet the driving force and mechanism for its spontaneous fluorophore synthesis are not established. Here we discover mutations that substantially slow the rate but not the yield of this posttranslational modification, d...

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Autors principals: Barondeau, David P., Putnam, Christopher D., Kassmann, Carey J., Tainer, John A., Getzoff, Elizabeth D.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC218721/
https://ncbi.nlm.nih.gov/pubmed/14523232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2133463100
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