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Method for isolating restriction- and modificationless mutants of Escherichia coli K-12.

A simple method is described for the selection and isolation of restriction- and modificationless mutants in Escherichia coli K-12 by using the following properties: (i) the temperature-sensitive repressor activity of phage lambdacI857; (ii) a mutant of lambda phage defective in integration and the...

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Dades bibliogràfiques
Publicat a:J Bacteriol
Autor principal: Del Giudice, L
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1979
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218501/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/368038/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.137.1.673-676.1979
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