Method for isolating restriction- and modificationless mutants of Escherichia coli K-12.
A simple method is described for the selection and isolation of restriction- and modificationless mutants in Escherichia coli K-12 by using the following properties: (i) the temperature-sensitive repressor activity of phage lambdacI857; (ii) a mutant of lambda phage defective in integration and the...
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| Udgivet i: | J Bacteriol |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1979
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218501/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/368038/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.137.1.673-676.1979 |
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