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Genetic transformation of Bacillus brevis 47, a protein-secreting bacterium, by plasmid DNA.

A method has been developed for introducing plasmid DNA into Bacillus brevis 47, a protein-secreting bacterium. Treatment of B. brevis 47 cells with 50 mM Tris-hydrochloride buffer of alkaline pH was effective for inducing DNA uptake competence. In the presence of polyethylene glycol, the Tris-treat...

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Bibliografiske detaljer
Main Authors: Takahashi, W, Yamagata, H, Yamaguchi, K, Tsukagoshi, N, Udaka, S
Format: Artigo
Sprog:Inglês
Udgivet: 1983
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC217958/
https://ncbi.nlm.nih.gov/pubmed/6358189
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