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Genetic transformation of Bacillus brevis 47, a protein-secreting bacterium, by plasmid DNA.

A method has been developed for introducing plasmid DNA into Bacillus brevis 47, a protein-secreting bacterium. Treatment of B. brevis 47 cells with 50 mM Tris-hydrochloride buffer of alkaline pH was effective for inducing DNA uptake competence. In the presence of polyethylene glycol, the Tris-treat...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Takahashi, W, Yamagata, H, Yamaguchi, K, Tsukagoshi, N, Udaka, S
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1983
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC217958/
https://ncbi.nlm.nih.gov/pubmed/6358189
Tagiau: Ychwanegu Tag
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