Genetic transformation of Bacillus brevis 47, a protein-secreting bacterium, by plasmid DNA.

A method has been developed for introducing plasmid DNA into Bacillus brevis 47, a protein-secreting bacterium. Treatment of B. brevis 47 cells with 50 mM Tris-hydrochloride buffer of alkaline pH was effective for inducing DNA uptake competence. In the presence of polyethylene glycol, the Tris-treat...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Takahashi, W, Yamagata, H, Yamaguchi, K, Tsukagoshi, N, Udaka, S
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1983
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217958/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6358189/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.156.3.1130-1134.1983
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