Electron microscopy of frozen-hydrated bacteria.

Amorphous, unstained, frozen-hydrated sections of bacteria provide a faithful high-resolution image of procaryotic cells. Conventional preparation artifacts due to fixation, staining, and dehydration are nonexistent. Freezing damage is avoided by using glucose as a cryoprotectant. Cutting damage on...

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Hauptverfasser: Dubochet, J, McDowall, A W, Menge, B, Schmid, E N, Lickfeld, K G
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1983
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC217690/
https://ncbi.nlm.nih.gov/pubmed/6408064
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