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Quantitative analysis of the binding affinity of poly(ADP-ribose) to specific binding proteins as a function of chain length

Poly(ADP-ribose) (PAR) is synthesized by poly(ADP-ribose) polymerases in response to genotoxic stress and interacts non-covalently with DNA damage checkpoint and repair proteins. Here, we present a variety of techniques to analyze this interaction in terms of selectivity and affinity. In vitro synth...

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Detalhes bibliográficos
Main Authors: Fahrer, Jörg, Kranaster, Ramon, Altmeyer, Matthias, Marx, Andreas, Bürkle, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2175335/
https://ncbi.nlm.nih.gov/pubmed/17991682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm944
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