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Copy Number Mutations (Cop(−)) of the Plasmid Containing the Replication Origin (oriC) of the Escherichia coli Chromosome: Lethal Effect of the Cop Region Cloned onto a High-Copy-Number Vector on Host Cells

High-copy-number mutants were isolated from an oriC plasmid. They carried insertion mutations within a region (about 470 base pairs) near the uncB gene. When a segment containing this region was cloned onto a high-copy-number plasmid, such a plasmid could be maintained as an intact form only when it...

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Vydáno v:J Bacteriol
Hlavní autoři: Yamaguchi, Kazuo, Yamaguchi, Mitsuyo
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1983
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217408/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6294061/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.153.1.550-554.1983
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