Transformation of intact yeast cells treated with alkali cations.

Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl. The optimum incubation time was 1 h, and the...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Ito, H, Fukuda, Y, Murata, K, Kimura, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1983
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217353/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6336730/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.153.1.163-168.1983
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