Transformation of intact yeast cells treated with alkali cations.
Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl. The optimum incubation time was 1 h, and the...
Gardado en:
| Publicado en: | J Bacteriol |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1983
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217353/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6336730/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.153.1.163-168.1983 |
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