Purification and properties of deoxyribonucleic acid polymerase from Bacillus stearothermophilus.

Deoxyribonucleic acid polymerase I was purified from Bacillus stearothermophilus to 50 to 70% homogeneity. Its molecular weight was 76,000. The enzyme was insensitive to sulfhydryl blocking agents and showed maximal activity at 60 degrees C, pH 8 to 9, 0.25 M KCl, and 0.02 M MgSO4. The rate of heat...

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Bibliographic Details

Published in:	J Bacteriol
Main Authors:	Kaboev, O K, Luchkina, L A, Akhmedov, A T, Bekker, M L
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1981
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217239/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7462144/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.145.1.21-26.1981
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