Purification and properties of deoxyribonucleic acid polymerase from Bacillus stearothermophilus.
Deoxyribonucleic acid polymerase I was purified from Bacillus stearothermophilus to 50 to 70% homogeneity. Its molecular weight was 76,000. The enzyme was insensitive to sulfhydryl blocking agents and showed maximal activity at 60 degrees C, pH 8 to 9, 0.25 M KCl, and 0.02 M MgSO4. The rate of heat...
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| 發表在: | J Bacteriol |
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| Principais autores: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
1981
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217239/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7462144/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.145.1.21-26.1981 |
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