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Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

We have employed the technique of gene fusion to fuse the LacZ gene encoding the cytoplasmic enzyme beta-galactosidase with the malE gene encoding the periplasmic maltose binding protein (MBP). Strains were obtained which synthesize malE-lacZ hybrid proteins of various sizes. These proteins have, at...

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Dettagli Bibliografici
Pubblicato in:J Bacteriol
Autori principali: Bassford, P J, Silhavy, T J, Beckwith, J R
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1979
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216822/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/110778/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.139.1.19-31.1979
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