Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.
We have employed the technique of gene fusion to fuse the LacZ gene encoding the cytoplasmic enzyme beta-galactosidase with the malE gene encoding the periplasmic maltose binding protein (MBP). Strains were obtained which synthesize malE-lacZ hybrid proteins of various sizes. These proteins have, at...
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| Pubblicato in: | J Bacteriol |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
1979
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216822/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/110778/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.139.1.19-31.1979 |
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