Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

We have employed the technique of gene fusion to fuse the LacZ gene encoding the cytoplasmic enzyme beta-galactosidase with the malE gene encoding the periplasmic maltose binding protein (MBP). Strains were obtained which synthesize malE-lacZ hybrid proteins of various sizes. These proteins have, at...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Bassford, P J, Silhavy, T J, Beckwith, J R
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1979
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216822/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/110778/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.139.1.19-31.1979
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