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Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

We have employed the technique of gene fusion to fuse the LacZ gene encoding the cytoplasmic enzyme beta-galactosidase with the malE gene encoding the periplasmic maltose binding protein (MBP). Strains were obtained which synthesize malE-lacZ hybrid proteins of various sizes. These proteins have, at...

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Bibliografski detalji
Glavni autori: Bassford, P J, Silhavy, T J, Beckwith, J R
Format: Artigo
Jezik:Inglês
Izdano: 1979
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC216822/
https://ncbi.nlm.nih.gov/pubmed/110778
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