Biochemical Characterization of SFC-1, a Class A Carbapenem-Hydrolyzing β-Lactamase

The carbapenem-hydrolyzing β-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penic...

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Detalhes bibliográficos
Main Authors: Fonseca, Fátima, Sarmento, Ana Cristina, Henriques, Isabel, Samyn, Bart, van Beeumen, Jozef, Domingues, Pedro, Domingues, Maria Rosário, Saavedra, Maria José, Correia, António
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2007
Assuntos:
Mechanisms of Resistance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2168006/
https://ncbi.nlm.nih.gov/pubmed/17875998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00491-07
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