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Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.

We have physically and genetically characterized 20 symbiotic and 20 auxotrophic mutants of Rhizobium meliloti, the nitrogen-fixing symbiont of alfalfa (Medicago sativa), isolated by transposon Tn5 mutagenesis. A "suicide plasmid" mutagenesis procedure was used to generate TN-5-induced mutants, and...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Meade, H M, Long, S R, Ruvkun, G B, Brown, S E, Ausubel, F M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216598/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6274841/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.149.1.114-122.1982
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