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Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.

We have physically and genetically characterized 20 symbiotic and 20 auxotrophic mutants of Rhizobium meliloti, the nitrogen-fixing symbiont of alfalfa (Medicago sativa), isolated by transposon Tn5 mutagenesis. A "suicide plasmid" mutagenesis procedure was used to generate TN-5-induced mutants, and...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Meade, H M, Long, S R, Ruvkun, G B, Brown, S E, Ausubel, F M
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1982
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216598/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6274841/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.149.1.114-122.1982
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