Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.

We have physically and genetically characterized 20 symbiotic and 20 auxotrophic mutants of Rhizobium meliloti, the nitrogen-fixing symbiont of alfalfa (Medicago sativa), isolated by transposon Tn5 mutagenesis. A "suicide plasmid" mutagenesis procedure was used to generate TN-5-induced mut...

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Bibliographic Details
Main Authors: Meade, H M, Long, S R, Ruvkun, G B, Brown, S E, Ausubel, F M
Format: Artigo
Language:Inglês
Published: 1982
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC216598/
https://ncbi.nlm.nih.gov/pubmed/6274841
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