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Construction and characterization of a deletion mutant lacking micF, a proposed regulatory gene for OmpF synthesis in Escherichia coli.

A method is presented for the construction of a deletion mutant lacking the micF gene, which has been proposed to negatively regulate expression of the ompF gene. The method includes (i) construction of a temperature-sensitive plasmid containing a chromosomal fragment that carries both flanking regi...

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Vydáno v:J Bacteriol
Hlavní autoři: Matsuyama, S, Mizushima, S
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1985
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215904/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3888961/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.3.1196-1202.1985
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