Construction and characterization of a deletion mutant lacking micF, a proposed regulatory gene for OmpF synthesis in Escherichia coli.
A method is presented for the construction of a deletion mutant lacking the micF gene, which has been proposed to negatively regulate expression of the ompF gene. The method includes (i) construction of a temperature-sensitive plasmid containing a chromosomal fragment that carries both flanking regi...
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| Опубликовано в:: | J Bacteriol |
|---|---|
| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
1985
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215904/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3888961/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.3.1196-1202.1985 |
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