Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.

Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombina...

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Main Authors: Smith, M D, Clewell, D B
Format: Artigo
Sprog:Inglês
Udgivet: 1984
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC215827/
https://ncbi.nlm.nih.gov/pubmed/6094500
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