Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene.
A major Bacillus subtilis 168S autolysin (N-acetylmuramoyl-L-alanine amidase [EC 3.5.1.28]) was purified and then cleaved with cyanogen bromide. The N-terminal amino acid sequence of one of the resultant peptides was determined in order to make synthetic oligonucleotides. A 2.5-kb EcoRI fragment was...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209238/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1682302/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.22.7304-7312.1991 |
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