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Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene.

A major Bacillus subtilis 168S autolysin (N-acetylmuramoyl-L-alanine amidase [EC 3.5.1.28]) was purified and then cleaved with cyanogen bromide. The N-terminal amino acid sequence of one of the resultant peptides was determined in order to make synthetic oligonucleotides. A 2.5-kb EcoRI fragment was...

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Bibliografiske detaljer
Main Authors: Kuroda, A, Sekiguchi, J
Format: Artigo
Sprog:Inglês
Udgivet: 1991
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC209238/
https://ncbi.nlm.nih.gov/pubmed/1682302
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