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Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments.

Deletion of an essential gene in Escherichia coli was accomplished by transformation of linear DNA fragments that have a Kanr gene segment flanked by sequences homologous to closely spaced regions on the E. coli chromosome. Selection for a double crossover within homologous sequences can effectively...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Jasin, M, Schimmel, P
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1984
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC215717/
https://ncbi.nlm.nih.gov/pubmed/6086588
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