Selection of lac gene fusions in vivo: ompR-lacZ fusions that define a functional domain of the ompR gene product.
We describe a simple method for selecting Escherichia coli mutants that carry gene fusions between a cloned gene and lacZ. We test this technique with the ompR gene, which codes for a positive regulatory factor in porin synthesis. A number of OmpR-LacZ hybrid proteins are examined, and several unusu...
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| Pubblicato in: | J Bacteriol |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
1984
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215709/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6086585/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.2.750-756.1984 |
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