Protoplast transformation of glutamate-producing bacteria with plasmid DNA.
A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during grow...
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| Published in: | J Bacteriol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1984
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215630/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6145700/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.306-311.1984 |
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