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Protoplast transformation of glutamate-producing bacteria with plasmid DNA.

A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during grow...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Katsumata, R, Ozaki, A, Oka, T, Furuya, A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1984
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215630/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6145700/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.306-311.1984
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