Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria.

The conjugative transposon Tn916 (15 kilobases), originally identified in Streptococcus faecalis DS16, has been cloned as an intact element on the pBR322-derived vector pGL101 in Escherichia coli. The EcoRI F' (EcoRI F::Tn916) fragment of pAM211 (pAD1::Tn916) was cloned into the single EcoRI si...

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Detaylı Bibliyografya
Asıl Yazarlar: Gawron-Burke, C, Clewell, D B
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1984
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC215615/
https://ncbi.nlm.nih.gov/pubmed/6330031
Etiketler: Etiketle
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