Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria.
The conjugative transposon Tn916 (15 kilobases), originally identified in Streptococcus faecalis DS16, has been cloned as an intact element on the pBR322-derived vector pGL101 in Escherichia coli. The EcoRI F' (EcoRI F::Tn916) fragment of pAM211 (pAD1::Tn916) was cloned into the single EcoRI site of...
Gardado en:
| Publicado en: | J Bacteriol |
|---|---|
| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1984
|
| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215615/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330031/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.214-221.1984 |
| Tags: |
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!
|