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Construction in vitro of a cloned nar operon from Escherichia coli.

To clone the nar operon of Escherichia coli without an effective selection procedure for the nar+ phenotype, a strategy utilizing nar::Tn5 mutants was employed. Partial segments of the nar operon containing Tn5 insertions were cloned into plasmid pBR322 by using the transposon resistance character f...

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Pubblicato in:J Bacteriol
Autori principali: Rondeau, S S, Hsu, P Y, DeMoss, J A
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1984
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215607/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330027/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.159-166.1984
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