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Cloning genes for proline biosynthesis from Neisseria gonorrhoeae: identification by interspecific complementation of Escherichia coli mutants.

DNA from Neisseria gonorrhoeae KH45 was partially digested with Sau3A and inserted into the BamHI site of the cloning vector pLES2 . After introduction into Escherichia coli JM83 by transformation, two different size classes of plasmids were isolated that could complement the proAB deletion of JM83...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Stein, D C, Silver, L E, Clark, V L, Young, F E
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1984
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215485/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6327618/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.158.2.696-700.1984
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