Cloning genes for proline biosynthesis from Neisseria gonorrhoeae: identification by interspecific complementation of Escherichia coli mutants.

DNA from Neisseria gonorrhoeae KH45 was partially digested with Sau3A and inserted into the BamHI site of the cloning vector pLES2 . After introduction into Escherichia coli JM83 by transformation, two different size classes of plasmids were isolated that could complement the proAB deletion of JM83...

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Bibliographic Details
Main Authors: Stein, D C, Silver, L E, Clark, V L, Young, F E
Format: Artigo
Language:Inglês
Published: 1984
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC215485/
https://ncbi.nlm.nih.gov/pubmed/6327618
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