Aerobic purification of hydrogenase from Rhizobium japonicum by affinity chromatography.

We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Stults, L W, Moshiri, F, Maier, R J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1986
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215196/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3519580/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.166.3.795-800.1986
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