Aerobic purification of hydrogenase from Rhizobium japonicum by affinity chromatography.
We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1986
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215196/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3519580/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.166.3.795-800.1986 |
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