Generation of up-regulated allosteric variants of potato ADP-glucose pyrophosphorylase by reversion genetics

Mutagenesis of the large subunit (LS) of the potato ADP-glucose pyrophosphorylase generated an enzyme, P52L, that was insensitive to 3-phosphoglycerate (3-PGA). To identify additional residues involved in 3-PGA interaction, we subjected P52L LS DNA to a second round of mutagenesis and identified sec...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Greene, Thomas W., Kavakli, Ibrahim Halil, Kahn, Michael L., Okita, Thomas W.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1998
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC21507/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9707646/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.17.10322
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