Generation of up-regulated allosteric variants of potato ADP-glucose pyrophosphorylase by reversion genetics

Mutagenesis of the large subunit (LS) of the potato ADP-glucose pyrophosphorylase generated an enzyme, P52L, that was insensitive to 3-phosphoglycerate (3-PGA). To identify additional residues involved in 3-PGA interaction, we subjected P52L LS DNA to a second round of mutagenesis and identified sec...

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Main Authors: Greene, Thomas W., Kavakli, Ibrahim Halil, Kahn, Michael L., Okita, Thomas W.
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 1998
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC21507/
https://ncbi.nlm.nih.gov/pubmed/9707646
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