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Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis.

The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (mol...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Pugsley, A P, Schwartz, M
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1983
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215057/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6352670/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.156.1.109-114.1983
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